picrosirius red  (StatLab Medical Products Inc)

Journal: bioRxiv

Article Title: ROCK2 inhibition has a dual role in reducing ECM remodelling and cell growth, while impairing migration and invasion

doi: 10.1101/2025.07.21.666015

Figure Lengend Snippet: ROCK2 inhibition with GV101 reduces CAF-mediated remodelling of pre-existing collagen in murine and human models of TNBC, also see Figures S1 and S2 (A) Schematic of collagen contraction assays utilising CAFs isolated from end-stage MMTV-PyMT tumours. (B) Representative images of PyMT CAF matrices treated with DMSO, GV101 or Fasudil at days 3 and 12 of contraction (i) and quantification of PyMT CAF organotypic matrix size over a 12-day contraction period (ii) and on day 12 (iii). (C) Representative SHG images of PyMT CAF matrices to assess collagen I (top panel, magenta), picrosirius red staining to assess fibrillar collagen (middle panel) and polarised light birefringence imaging to assess collagen bundling and density (bottom panel). (D) Quantification of unconfined compression analysis to assess Young’s modulus. (E-G) Quantification of PyMT CAF matrices; SHG peak signal intensity (E), picrosirius red staining coverage (F) and total birefringence signal (G). (H) Schematic of collagen contraction assays utilising CAFs isolated from human TNBC patients. (I) Representative images of human CAF matrices treated with DMSO, GV101 or Fasudil at days 3 and 12 of contraction (i) and quantification of CAF organotypic matrix size over a 12-day contraction period (ii) and on day 12 (iii). (J) Representative SHG images of human CAF matrices to assess collagen I (top panel, magenta), picrosirius red staining to assess fibrillar collagen (middle panel) and polarised light birefringence imaging to assess collagen bundling and density (bottom panel). (K) Quantification of unconfined compression analysis to assess matrix Young’s modulus. (L-N) Quantification of human CAF matrices; SHG peak signal intensity (L), picrosirius red staining coverage (M) and total birefringence signal (N). Data represents mean ± SEM of three individual repeats. p-values were determined using One-sample t and Wilcoxon test for normalised data and for remaining data a One-way ANOVA with multiple comparisons was used. ns = not significant (p≥0.05), * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001. Scale bar = 1cm (B and I), 100µm (C and J).

Article Snippet: Slides were then incubated in 0.02% phosphomolybdic acid for 2 minutes and briefly washed in water before a 2 hour incubation in 0.1% Picrosirius Red solution (Australian Biostain solution).

Techniques: Inhibition, Isolation, Staining, Imaging

Journal: bioRxiv

Article Title: ROCK2 inhibition has a dual role in reducing ECM remodelling and cell growth, while impairing migration and invasion

doi: 10.1101/2025.07.21.666015

Figure Lengend Snippet: (A) Schematic of cell-derived matrix (CDM) production assays where both PyMT and human CAFs are treated with ascorbic acid to stimulate matrix production in the presence of DMSO, GV101 or Fasudil. (B) Representative images of PyMT CAF derived CDMs showing SHG imaging (top panel, magenta) and picrosirius red stained matrices (bottom panel). (C) Quantification of peak SHG signal (i) and picrosirius red staining coverage for PyMT CAF generated CDMs (ii). (D) Representative images of human CAF derived CDMs showing SHG imaging (top panel, magenta) and picrosirius red stained matrices (bottom panel). (E) Quantification of peak SHG signal (i) and picrosirius red coverage (ii) for human CAF generated CDMs. Data represents mean ± SEM of three individual repeats. p-values were determined using One-sample t and Wilcoxon test for normalised data and for remaining data a One-way ANOVA with multiple comparisons was used. ns = not significant (p≥0.05), * = p<0.05, ** = p<0.01. Scale bar = 100μm.

Article Snippet: Slides were then incubated in 0.02% phosphomolybdic acid for 2 minutes and briefly washed in water before a 2 hour incubation in 0.1% Picrosirius Red solution (Australian Biostain solution).

Techniques: Derivative Assay, Imaging, Staining, Generated

Journal: bioRxiv

Article Title: Controlled decorin delivery from injectable microgels promotes scarless vocal fold repair

doi: 10.1101/2025.06.25.661429

Figure Lengend Snippet: ( A ) Schematic and endoscopy images of endoscopy-guided intracordal injection and vocal fold (VF) scarring rat model. ( B ) Hematoxylin & eosin (H&E) and ( C ) hyaluronan binding protein (HABP) histology of rat larynges injected with blank hyaluronic acid MGs show successful intracordal injection of the underlying vocalis muscle. Dotted outlines are drawn around injected microgels. ( D ) H&E staining of mid-coronal vocal fold sections suggests a denser extracellular matrix in scarred VFs left untreated or injected with blank microgels relative to healthy rats and injured rats injected with DCN-loaded microgels at 7 days post-injury. ( E&G ) Picrosirius red staining visualized by polarized light microscopy show that VF scarring is characterized by significant collagen accumulation, which is unaffected by injection of blank microgels. DCN-loaded microgels were sufficient to prevent collagen accumulation. Dotted outlines drawn around the VF lamina propria for each representative image. ( F&H ) DCN-MGs mitigates persistent myofibroblast activation in vivo as evidenced by reduced α-SMA (myofibroblast marker) immunostaining in vivo. Dotted outlines drawn around the VF lamina propria for each representative image. *=p<0.05, **=p<0.01

Article Snippet: Picrosirius red was purchased from StatLab (McKinney, TX).

Techniques: Injection, Binding Assay, Staining, Light Microscopy, Activation Assay, In Vivo, Marker, Immunostaining

Journal: Cell Reports Medicine

Article Title: Cross-species tropism of AAV.CPP.16 in the respiratory tract and its gene therapies against pulmonary fibrosis and viral infection

doi: 10.1016/j.xcrm.2025.102144

Figure Lengend Snippet: An AAV.CPP.16-mediated gene therapy for idiopathic pulmonary fibrosis (A) Schematic diagram illustrating design of a bifunctional fusion protein that inhibits VEGF and TGF-β1 and construction of an AAV vector expressing such a protein. (B) Experimental design for testing the AAV.CPP.16 gene therapy (CPP.16-IPF trap) in a bleomycin-induced IPF mouse model. i.t., intratracheal; inhal., Inhaled; i.g., intragastric. (C) Representative H&E staining on lung sections in mice with different treatments. Scale bar: 200 μm for low-magnification images and 50 μm for enlarged images. (D) Quantification of lung cell areas in H&E-stained lung sections. n = 3–6 per group, mean ± SEM, one-way ANOVA with Tukey’s post-test. (E) Representative Masson, picrosirius red, and αSMA immunohistochemical staining of lung sections in mice with different treatments. Masson (blue) and picrosirius red (red) stainings label collagen expression. Scale bar: 200 μm. (F) Quantification of fibrotic areas on lung sections. n = 3–6 per group, mean ± SEM, one-way ANOVA with Tukey’s post-test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. PBS, phosphate-buffered saline; BLM, bleomycin; IPF, idiopathic pulmonary fibrosis; PFD, pirfenidone; H&E, hematoxylin and eosin.

Article Snippet: Picrosirius red staining , Servicebio , Cat# G1078.

Techniques: Plasmid Preparation, Expressing, Staining, Immunohistochemical staining, Saline

Journal: Scientific Reports

Article Title: Cell spheroid micromechanics under large deformations

doi: 10.1038/s41598-025-03676-3

Figure Lengend Snippet: ( a ) F-ε z fitting before (red) and after (green) apparent Poisson’s ratio correction. ( b ) Lateral strain ε x as a function of compressive strain ε z . The red and green curves represent the computed lateral strain assuming incompressibility and compressibility, respectively. The black dots were obtained from the CS image segmentation and shape analysis. Error bars represent standard deviation ( n = 6), ( c ) Sensitivity of the apparent modulus calculated at 0.5 μm/s displacement rate to variations in apparent Poisson’s ratio, ( d ) Confocal fluorescence images of a CS composed of human primary cardiac fibroblasts, stained with BioTracker 488 (cell body, green), Hoechst 33342 (cell nuclei, blue) and Picrosirius red (collagen fibrils, red). Showing a maximum intensity projection of a 70 μm z-stack.

Article Snippet: CSs were stained using cell body BioTracker green stain (Sigma-Aldrich, 5μM), Hoechst 33342 stain (Thermo Fisher Scientific, 10 μg/mL) and Picrosirius Red (Morphisto, 0.1% Sirius red in saturated picric acid), according to manufacturer’s protocols.

Techniques: Standard Deviation, Fluorescence, Staining